Illegal NgoMIV site found at 462 Illegal AgeI site found at 154 Illegal AgeI site found at 675
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 595
Design Notes
The original pobA/R operon consists of six main components: pobA, pobA RBS, pobA/R dual-directional promoter, pobR operator, pobR RBS, and pobR. The pobA gene encodes an unnecessary enzyme that breaks down 4-HBA. Therefore, we remove the pobA and pobA RBS components but retain the entire promoter, pobR RBS, and pobR.
Regarding the original digitizer, we eliminate the XylS system, which includes the XylS protein and its corresponding promoter. We then combine these two sections and anticipate that the new chimeric digitizer will function properly.
We use GFP to test our new chimeric digitizer. If it functions well, we will replace GFP with luxI to establish our bio-sensor.
===Source===
DiMarco, A. A., Averhoff, B., & Ornston, L. N. (1993). Identification of the transcriptional activator pobR and characterization of its role in the expression of pobA, the structural gene for p-hydroxybenzoate hydroxylase in Acinetobacter calcoaceticus. 175(14), 4499-4506. doi:doi:10.1128/jb.175.14.4499-4506.1993
===References===
DiMarco, A. A., Averhoff, B., & Ornston, L. N. (1993). Identification of the transcriptional activator pobR and characterization of its role in the expression of pobA, the structural gene for p-hydroxybenzoate hydroxylase in Acinetobacter calcoaceticus. 175(14), 4499-4506. doi:doi:10.1128/jb.175.14.4499-4506.1993
