Composite

Part:BBa_K4724083

Designed by: Xinyao Li   Group: iGEM23_NJTech-China-A   (2023-10-09)
Revision as of 19:47, 10 October 2023 by Yaoyaowuqi (Talk | contribs)


DsbA-LSPETase

Insert the signal peptide DsbA gene sequence into the vector pET-22b (+) - LSPET-12, and construct a recombinant plasmid using Gibson assembly method to promote the soluble expression of the original target fragment LSPET protein.

Characterize the results


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal NgoMIV site found at 442
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None