Part:BBa_K4579033
pBTK1028 assembly backbone
Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin.
The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix.
For literature describing the BTK and YTK standards, please see the References section on the design page for this part.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal NheI site found at 2071
Illegal NotI site found at 182
Illegal NotI site found at 2225 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal BglII site found at 1070 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 406
Illegal AgeI site found at 1661
Illegal AgeI site found at 1985 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 346
Illegal SapI site found at 556
| None |