DNA

Part:BBa_K4765113

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 08:48, 9 October 2023 by Siliang Zhan (Talk | contribs) (→‎Characterization)


Twister P1 + T7_RBS + H. ex mtSSB + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and ribozyme-assisted polycistronic co-expression system：pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.

Usage and Biology

We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112(ribozyme+T7_RBS+SAHS 33020+stem-loop) to increase the survival rate of E.coli under desiccation.

Characterization

Get details in BBa_K4765016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409


Reference

[edit]
Categories
//collections
Parameters
protein