DNA

Part:BBa_K4642037:Design

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-10-09)
Revision as of 08:34, 9 October 2023 by RYapa (Talk | contribs)


hsa-miR-145 Probe 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to design probes for miRPA, we used NUPACK’s design functions in its API to find probes which would bind to the miRNAs, but have overhangs which didn’t bind within themselves, to ensure primers could easily anneal to them. We then tested, using NUPACK, the specificity of these probes and found that they had a less than 1% likelihood of binding to the closest human miRNA homologs in the correct shape. This demonstrated the increase in specificity that using miRPA could add to our test.


Source

Custom designed in-silico using NUPACK API.

References