Composite

Part:BBa_K4604026

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-08)
Revision as of 19:31, 8 October 2023 by HannahSwientek (Talk | contribs)


piG_K12BSa (LacIprom_riboK12_lacI_trcProm_sfGFP)

piG_K12BSa consists of the LacI promoter, the AdoCbl riboswitch from E. coli K12, lacI and the trc promoter. The trc promoter can be induced with IPTG. This BioBrick is a sensor for AdoCbl (a bioavailable form of B12) with a variable marker gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 215
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None