Part:BBa_K200028

Protease resistant PAH

The Homo sapiens endogenous enzyme is synthesised by the liver. However, as part of the Imperial College iGEM 2009 project, [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator], the enzyme is released in the intestine of the individual. The presence of proteases in the intestine was therefore a serious problem which would jeopardise the viability of the curative enzyme. We thus performed site directed mutagenesis in order to alter a serine residue (Ser16) to a glutamine. This change has previously been correlated with resistance against intestinal proteases#PRPAH1. In fact, it is thought that phosphorylation of the serine 16 is responsible for the resistance to proteases#PRPAH2. This phosphorylated state is mimicked by replacing the serine with a glutamine residue.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 287
  Illegal BamHI site found at 814
  Illegal XhoI site found at 524
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

<biblio>

1. PRPAH1 pmid=7887915
2. PRPAH2 pmid=8573072

</biblio>