Composite

Part:BBa_K4800036

Designed by: Yao Yao   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 07:08, 11 October 2023 by ZQM 0907 (Talk | contribs)

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pRSFDuet-MAB4714

We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MAB4714 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MAB4714.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7185
    Illegal PstI site found at 7197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7233
    Illegal PstI site found at 7197
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3574
    Illegal BamHI site found at 7179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7185
    Illegal PstI site found at 7197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7185
    Illegal PstI site found at 7197
    Illegal NgoMIV site found at 4052
    Illegal NgoMIV site found at 4354
    Illegal NgoMIV site found at 4544
    Illegal NgoMIV site found at 4907
    Illegal NgoMIV site found at 5962
    Illegal NgoMIV site found at 6341
    Illegal NgoMIV site found at 6566
    Illegal AgeI site found at 3872
    Illegal AgeI site found at 4091
    Illegal AgeI site found at 5114
    Illegal AgeI site found at 5834
    Illegal AgeI site found at 6388
    Illegal AgeI site found at 7608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7429
    Illegal BsaI site found at 7681
    Illegal SapI site found at 660


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Categories
Parameters
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