Coding

Part:BBa_K4879001:Design

Designed by: Rohaneshwar Manikandan   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 12:15, 11 October 2023 by Sauron 3791 (Talk | contribs)

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JcFATB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 831
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 831
    Illegal PstI site found at 50
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 831
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 831
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 831
    Illegal PstI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.

https://www.ncbi.nlm.nih.gov/gene/105639285

References

Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209