Composite

Part:BBa_K4800033

Designed by: Qiming Zou   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 08:35, 8 October 2023 by ZQM 0907 (Talk | contribs)


pRSFDuet-MAB3367

Carboxylic acid reductase from Mycobacterium abscessus.The enzyme contains an adenylation domain, a phosphopantetheinyl binding domain, and a reductase domain, and requires activation by attachment of a phosphopantetheinyl group. The enzyme activates its substrate to an adenylate form, followed by a transfer to the phosphopantetheinyl binding domain. The resulting thioester is subsequently transferred to the reductase domain, where it is reduced to an aldehyde and released.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5168
    Illegal EcoRI site found at 6122
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7140
    Illegal PstI site found at 3651
    Illegal PstI site found at 4052
    Illegal PstI site found at 5345
    Illegal PstI site found at 6002
    Illegal PstI site found at 7152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5168
    Illegal EcoRI site found at 6122
    Illegal PstI site found at 3651
    Illegal PstI site found at 4052
    Illegal PstI site found at 5345
    Illegal PstI site found at 6002
    Illegal PstI site found at 7152
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5168
    Illegal EcoRI site found at 6122
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7140
    Illegal PstI site found at 3651
    Illegal PstI site found at 4052
    Illegal PstI site found at 5345
    Illegal PstI site found at 6002
    Illegal PstI site found at 7152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5168
    Illegal EcoRI site found at 6122
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7140
    Illegal PstI site found at 3651
    Illegal PstI site found at 4052
    Illegal PstI site found at 5345
    Illegal PstI site found at 6002
    Illegal PstI site found at 7152
    Illegal NgoMIV site found at 3644
    Illegal NgoMIV site found at 3868
    Illegal NgoMIV site found at 4262
    Illegal NgoMIV site found at 5389
    Illegal NgoMIV site found at 5926
    Illegal NgoMIV site found at 5930
    Illegal AgeI site found at 4327
    Illegal AgeI site found at 4391
    Illegal AgeI site found at 5084
    Illegal AgeI site found at 5747
    Illegal AgeI site found at 6349
    Illegal AgeI site found at 7563
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7384
    Illegal BsaI site found at 7636
    Illegal SapI site found at 660


[edit]
Categories
Parameters
None