Composite
Part:BBa_K4800033
Designed by: Qiming Zou Group: iGEM23_NJTech-China-B (2023-10-08)
pRSFDuet-MAB3367
Carboxylic acid reductase from Mycobacterium abscessus.The enzyme contains an adenylation domain, a phosphopantetheinyl binding domain, and a reductase domain, and requires activation by attachment of a phosphopantetheinyl group. The enzyme activates its substrate to an adenylate form, followed by a transfer to the phosphopantetheinyl binding domain. The resulting thioester is subsequently transferred to the reductase domain, where it is reduced to an aldehyde and released.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5168
Illegal EcoRI site found at 6122
Illegal XbaI site found at 1415
Illegal XbaI site found at 7140
Illegal PstI site found at 3651
Illegal PstI site found at 4052
Illegal PstI site found at 5345
Illegal PstI site found at 6002
Illegal PstI site found at 7152 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5168
Illegal EcoRI site found at 6122
Illegal PstI site found at 3651
Illegal PstI site found at 4052
Illegal PstI site found at 5345
Illegal PstI site found at 6002
Illegal PstI site found at 7152 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5168
Illegal EcoRI site found at 6122
Illegal XbaI site found at 1415
Illegal XbaI site found at 7140
Illegal PstI site found at 3651
Illegal PstI site found at 4052
Illegal PstI site found at 5345
Illegal PstI site found at 6002
Illegal PstI site found at 7152 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5168
Illegal EcoRI site found at 6122
Illegal XbaI site found at 1415
Illegal XbaI site found at 7140
Illegal PstI site found at 3651
Illegal PstI site found at 4052
Illegal PstI site found at 5345
Illegal PstI site found at 6002
Illegal PstI site found at 7152
Illegal NgoMIV site found at 3644
Illegal NgoMIV site found at 3868
Illegal NgoMIV site found at 4262
Illegal NgoMIV site found at 5389
Illegal NgoMIV site found at 5926
Illegal NgoMIV site found at 5930
Illegal AgeI site found at 4327
Illegal AgeI site found at 4391
Illegal AgeI site found at 5084
Illegal AgeI site found at 5747
Illegal AgeI site found at 6349
Illegal AgeI site found at 7563 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7384
Illegal BsaI site found at 7636
Illegal SapI site found at 660
[edit]
Categories
Parameters
None |