Part:BBa_K4591002

XylSmut

Improvement

The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.It was

Usage and Biology

XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding an`d protein dimerization. [1]

XylS can bind benzoic acid and various derivatives, but it cannot recognize PA and TPA.[2-5] So the Jiawei Li and Mario Roque Huanca Nina successfully make the directed evolution of XylS to generate new TFs that are capable of binding and responding to TPA and PA . Such XylS mutants could be used to construct whole-cell biosensors for fluorimetric detection of PA and TPA.

photo1 from ShiMing

When the TPA was detected by the Xylsmut, the P_m promotor would be actived and transcript the downstream fragments.[6]

In this year, we have designed a system of PET detection, attachment and degradation.In order to detect the TPA, the degradation products of the PET. The Xylsmut was chosen as the "sensor" of the TPA concentration, and the deGFP was also constructed in our circuit as the indicator of TPA.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]