Regulatory

Part:BBa_K4767001

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 07:13, 8 October 2023 by Airplan mod (Talk | contribs) (→‎Functional Parameters)


Pars

This part is an arsenic-responsive promoter that couples with the transcriptional regulator ArsR. It is from Escherichia coli genome which can response to the ArsR protein and block the transcription of downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

In 2023, CUG-China used this arsenic-responsive transcription system to detect arsenic. We placed gfp at downstream of the Pars -arsR system and demonstrated the As(Ⅲ) can bind the ArsR and drive the expression of gfp. As the figure shows, with an increase in arsenic concentrations, the fluorescence intensity increased in response.

img-1165.png
Figure 1. The fluorescence curves of Shewanella oneidensis MR1 cells with the arsenic-responsive transcription gene circuits.

References

Jia X, Bu R, Zhao T, et al. Sensitive and specific whole-cell biosensor for arsenic detection[J]. Applied and Environmental Microbiology, 2019, 85(11): e00694-19.

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Parameters
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