Part:BBa_K4591002
XylSmut
ZJUT-China 2023's Contribution
Usage and Biology
XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding an`d protein dimerization. [1]
XylS can bind benzoic acid and various derivatives, but it cannot recognize PA and TPA.[2-5] So the Jiawei Li and Mario Roque Huanca Nina successfully make the directed evolution of XylS to generate new TFs that are capable of binding and responding to TPA and PA . Such XylS mutants could be used to construct whole-cell biosensors for fluorimetric detection of PA and TPA.
photo1 from ShiMing
BBa_K3202001 When the TPA was detected by the Xylsmut, the Pm promotor would be actived and transcript the downstream fragments.[6]
In this year, we have designed a system of PET detection, attachment and degradation.In order to detect the <p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.It was
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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