Composite

Part:BBa_K4724075

Designed by: Xinyao Li   Group: iGEM23_NJTech-China-A   (2023-10-07)
Revision as of 19:11, 10 October 2023 by Yaoyaowuqi (Talk | contribs)


NusA-LSPET

We fused the NusA fusion tag to the N-terminus of LSPETase to prevent self-aggregation and aggregation by binding and isolating its target protein's prone-to-aggregate folding intermediates, thereby ensuring stable expression of the recombinant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal XhoI site found at 2344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None