Coding

Part:BBa_K4765019

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 03:01, 8 October 2023 by Siliang Zhan (Talk | contribs) (sequencing map)


XRCC1

contributed by Fudan iGEM 2023

Introduction

XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents. It collaborates with DNA ligase III, polymerase β, and poly (ADP-ribose) polymerase in base excision repair. It might also play a role in meiosis-related DNA processes. Although XRCC1 lacks enzymatic activity, it acts as a scaffold for repair enzymes, aiding in single-strand break repair, base excision repair, and nucleotide excision repair[1]. XRCC1's structure includes three domains, enabling interactions with various repair proteins. Additionally, it is involved in error-prone microhomology-mediated end joining repair of double-strand breaks, often leading to mutation-inducing deletions.

Usage and Biology

We heterologously expressed codon-optimized XRCC1 in E. coli, endowing it with anti-UV capability.

Characterization

Sequencing map

contributed by Fudan iGEM 2023
Figure1 Sequencing map of XRCC11 Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 685
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1735


Reference

  1. London R. E. (2015). The structural basis of XRCC1-mediated DNA repair. DNA repair, 30, 90–103. https://doi.org/10.1016/j.dnarep.2015.02.005
[edit]
Categories
//cds
Parameters
protein