Composite
Part:BBa_K4757999
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-10-07)
Synthetic expression cassette regulated by terepthalic acid and alkanes for PET and PE sensing
<img src="img_girl.jpg" alt="Girl in a jacket" width="500" height="600">
Expression cassette consinsting of XylS-K38R-L224Q/Pm promoter, activated by terepthalic acid, and AlkS-V760E/pAlkB activated by n-dodecane.
Gene expression controlled by the pAlkB promoter can be degraded with sRNA interference, expressed with Pm promoter. test 123
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4202
Illegal EcoRI site found at 4298 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4202
Illegal EcoRI site found at 4298
Illegal NotI site found at 1625 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4202
Illegal EcoRI site found at 4298
Illegal BglII site found at 212 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4202
Illegal EcoRI site found at 4298 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4202
Illegal EcoRI site found at 4298
Illegal NgoMIV site found at 934
Illegal NgoMIV site found at 2716
Illegal NgoMIV site found at 3187 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |