Composite

Part:BBa_K4759209

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 07:40, 12 October 2023 by JianghuaChen (Talk | contribs)


T7-RBS4-GST-linker-Olep

we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 890
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 177


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