Composite

Part:BBa_K4759206:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 07:38, 12 October 2023 by JianghuaChen (Talk | contribs) (→‎Design Notes)


T7-RBS4-TF-linker-OleP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 591
    Illegal AgeI site found at 1428
    Illegal AgeI site found at 1526
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 159
    Illegal SapI.rc site found at 807
    Illegal SapI.rc site found at 920
    Illegal SapI.rc site found at 1176


Design Notes

we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

Source

1

References