Coding

Part:BBa_K4604011

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)
Revision as of 16:19, 7 October 2023 by HannahSwientek (Talk | contribs)


MazF toxin

In this BioBrick we fused MazF together with a FLAG-tag using a 3X Alanine linker. This FLAG-tag can be used for protein detection in western blot. Used in Biobricks BBa_K4604017, BBa_K4604018, BBa_K4604019, BBa_K4604021, BBa_K4604022, BBa_K4604023, BBa_K4604024 and BBa_K4604025.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[1]Espah Borujeni, Amin et al. “Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.” Nucleic Acids Research 44 (2015): 1 - 13. [2]BRZOZOWSKA, Iwona; ZIELENKIEWICZ, Urszula. Regulation of toxin–antitoxin systems by proteolysis. Plasmid, 2013, 70. Jg., Nr. 1, S. 33-41.

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