Coding

Part:BBa_K4879000:Design

Designed by: Rohaneshwar Manikandan, Ishaan Chaudhary   Group: iGEM23_IISER-Pune-India   (2023-10-07)
Revision as of 15:21, 10 October 2023 by Sauron 3791 (Talk | contribs)


JcFATA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica. It also has silent mutations to make the part Type II Standard compatible.


Source

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References