Part:BBa_K4897002
BS DNA-322 (BS DNA 3.0) using in L-RCA for detecting P. acne
BS DNA-322 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element in reacting with the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers
Usage and Biology
Fig. 1. The process of BS DNA binding to P. acne DNA.
BS DNA-322 is the red line like a padlock. The black sequence represents the P. acne’s DNA specific to the binding. The red boxes represent the binding regions of the two ends of BS DNA-322 (notice that the two ends are not connected). The purple boxes represent the random sequences generated. The Blue box represents the amplification region for RCA.
BS DNA-322 would bind the bacterial DNA of P. acne and show great sensitivity and specificity. It can stably bind to the specific bacterial DNA of P. acne and quantitatively indicate the amount of P. acne in the amplification result.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 161
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 161
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 161
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 161
- 1000COMPATIBLE WITH RFC[1000]
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