Composite

Part:BBa_K4604016:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-06)
Revision as of 14:47, 7 October 2023 by HannahSwientek (Talk | contribs)


piG_01a (leaky_tetR_bluB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1647


Design Notes

Correct overhangs had to be designed for Golden Gate Cloning. The start codon of the bluB was changed to ATG to act as a start for translation in E. coli.


Source

Assebled via Golden Gate Cloning.

References