Composite
Part:BBa_K4800003
Designed by: Yao Yao Group: iGEM23_NJTech-China-B (2023-10-06)
Ptrc-sfp-MmCAR-YahK
(1)Ptrc is a promoter.(2)YahK is a acyl-CoA thioester hydrolase from Haemophilus influenzae.(3) Sfp is a phosphopantetheinyl transferase from Bacilus subtilis.(4) MmCAR is a Carboxylic acid reductase from Mycobacterium marinum.(5) YahK is a NADPH-dependent aldehyde reductase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1332
Illegal PstI site found at 1767
Illegal PstI site found at 1794
Illegal PstI site found at 3063
Illegal PstI site found at 3090
Illegal PstI site found at 3483
Illegal PstI site found at 3979
Illegal PstI site found at 4623 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1332
Illegal NheI site found at 4891
Illegal PstI site found at 1767
Illegal PstI site found at 1794
Illegal PstI site found at 3063
Illegal PstI site found at 3090
Illegal PstI site found at 3483
Illegal PstI site found at 3979
Illegal PstI site found at 4623 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1332
Illegal PstI site found at 1767
Illegal PstI site found at 1794
Illegal PstI site found at 3063
Illegal PstI site found at 3090
Illegal PstI site found at 3483
Illegal PstI site found at 3979
Illegal PstI site found at 4623 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1332
Illegal PstI site found at 1767
Illegal PstI site found at 1794
Illegal PstI site found at 3063
Illegal PstI site found at 3090
Illegal PstI site found at 3483
Illegal PstI site found at 3979
Illegal PstI site found at 4623
Illegal NgoMIV site found at 3591
Illegal NgoMIV site found at 3653
Illegal NgoMIV site found at 3870
Illegal NgoMIV site found at 4317
Illegal AgeI site found at 1497
Illegal AgeI site found at 2508
Illegal AgeI site found at 2805
Illegal AgeI site found at 3690
Illegal AgeI site found at 3699
Illegal AgeI site found at 4374 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |