Coding
Part:BBa_K4586032
Designed by: Mohamed Mohamed Saad Aboelghare Group: iGEM23_AFCM-Egypt (2023-10-05)
Revision as of 21:26, 11 October 2023 by Ahmed Mattar (Talk | contribs)
Anti CD19
Usage and Description
This part codes for the light and heavy chain scFv of anti CD19, that represent the extra cellular domain in syn notch receptor sensitive for CD19.
This figure show our Syn-Notch receptor which represent Anti-CD19 as our ligand binding domain (LBD).
Experimental Characterization
In order to amplify this DNA part, we used PCR amplification to reach the desired concentration to complete our experiments using specific forward and reverse primers, running the parts on gel electrophoresis as this part presents lane (p5) including lamb2p an anti-CD19 , and then we measured the specific concentration of the running part using Real-Time PCR as shown in the following figure.
We performed the double digestion method for this part in the prefix and suffix with its specific restriction enzyme and applied this part to gel electrophoresis as shown in the following figure lane(P10).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 444
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Categories
Parameters
None |