Coding

Part:BBa_K4579035

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-10-05)
Revision as of 23:30, 5 October 2023 by Alexakmorton (Talk | contribs)


pBTK205 - GFP coding sequence

Following the part type standard of the Bee Toolkit created by Leonard et al., pBTK205 is a type 3 part that consists of the coding sequence for GFPmut3, a green fluorescent protein.

The first four bases of this part correspond to a type 3 part forward prefix (TATG), and the last four bases of this part correspond to a type 3 part reverse suffix (GGAT). btk-overhangs-low-ish-res.png

For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


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Categories
Parameters
None