Plasmid_Backbone

Part:BBa_K4579034

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-10-05)
Revision as of 23:07, 5 October 2023 by Alexakmorton (Talk | contribs)


pBTK1001 part backbone

This is an entry vector part from the Bee Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly in the entry vector pBTK1028 (BBa_K4579033). The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening, but the sequence provided is that of the plasmid after the dropout has been digested out by BsmBI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
None