Part:BBa_K4768008

Split T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker

Part for Expression of the T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker ZipA

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 987
  Illegal XbaI site found at 40
  Illegal SpeI site found at 1147
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 987
  Illegal SpeI site found at 1147
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 987
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 987
  Illegal XbaI site found at 40
  Illegal SpeI site found at 1147
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 987
  Illegal XbaI site found at 40
  Illegal SpeI site found at 1147
  Illegal AgeI site found at 1200
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 21
  Illegal SapI.rc site found at 1836
  

xxxxxxxxxxxxxxxxxxadd part imagexxxxxxxxxxxxx

Introduction

Figure 1: NT7-ZipA-Pertuzumab structure.

The CALIPSO part BBa_K4768008 is composed of the N-terminal subunit of the T7 RNA polymerase (residues 1 to 180) fused to the anti-HER2 antibody Pertuzumab through an optimized linker sequence containing a transmembrane domain. This gene is under transcriptional control of an SP6 promoter and T7 terminator.

Construction

TXXXXXX.

Molecular Modeling

TXXXXXX.

Characterisation

TXXXXXX.

Conclusion and Perspectives

TXXXXXX.

References

1. article 1 xxxxxxxx
2. article 2 xxxxxxx