Coding

Part:BBa_K4759037:Experience

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)
Revision as of 16:36, 7 October 2023 by JianghuaChen (Talk | contribs) (→‎Applications of BBa_K4759037)

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Applications of BBa_K4759037

we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags

(SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of

Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

protein-tags.png

The application of protein tags

User Reviews

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