Part:BBa_K4882007

Mcl1 signal peptide-LqhIT2

This is an Mcl1 secretory signal peptide fused with an insect-specific toxin LqhIT2.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 145
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The Mcl1 signal peptide (Mcl1ss, https://parts.igem.org/Part:BBa_K4882002 BBa_K4882002]) is from the collagen-like protein MCL1 of Metarhizium anisopliae. Mcl1ss is an 18-amino acid secretory signal peptide at the N-terminus of the MCL1 protein.

LqhIT2 (BBa_K4882001) is a 61 amino acid-long scorpion depressant toxin from L. quinquestriatus hebraeus. Although the scorpion is dangerous to humans, LqhIT2 is not. LqhIT2 shows a high preference for insect voltage-gated sodium channels and is totally harmless to mammals.

To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene.