Regulatory
Part:BBa_K4882000
Designed by: Yuqi Fu Group: iGEM23_Hangzhou-SDG (2023-06-09)
Pmcl1 (short), a truncated version of hemolymph inducible promoter from Metarhizium anisopliae
The Pmcl1 promoter is responsible for regulating the collagen-like protein MCL1 in M. anisopliae. Pmcl1 is a hemolymph inducible promoter that turns on only in insect hemolymph (Wang et al., 2006). iGEM16_NYMU-Taipei registered the full-length Pmcl1 as BBa_K2040100. Compared to the full-length Pmcl1 (2764bp), a truncated, shorter version of Pmcl1 (1586bp) can lead to a twofold increase in downstream gene expression (Kanjo et al., 2019).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 489
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 45
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |