Coding

Part:BBa_K4790073

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-10-04)
Revision as of 10:57, 12 October 2023 by Xzqqq (Talk | contribs)


node59-Y6A

ASR1(node59) is constructed on pET-22b(+) vector by Gibson Assembly method. DNA frament node 59 was subcloned into RBS site between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicilin resistance gene..

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None