RBS

Part:BBa_B0034:Experience

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.   Group: Antiquity   (2003-01-31)
Revision as of 12:44, 19 May 2010 by Maven (Talk | contribs) (→‎User Reviews)

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Applications of BBa_B0034

User Reviews

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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Aberdeen_Scotland 2009

Our miniprep, double digest and gel worked properly. We used this part in fusion PCR for building our biobrick BBa_K182102. The fusion PCR, cloning and sequence were all correct.

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iGEM Groningen 2009

The ligation of part BBa_K190028 behind the RBS was successful, confirmed by gel (correct vector size after digestion with EcoRI and PstI) and sequencing with VF2 primer. We used this part in combination with several genes for building our biobricks e.g. BBa_K190061.

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maven

Defining efficiency for an RBS seems misleading, since RBS efficiency can only be considered in context of its upstream and downstream sequences as this [http://www.nature.com/nbt/journal/v27/n10/abs/nbt.1568.html|article] demonstrated.


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