Coding

Part:BBa_K4593002:Design

Designed by: Zixuan Zhou   Group: iGEM23_BNDS-China   (2023-09-26)
Revision as of 17:34, 3 October 2023 by Jianfei Song (Talk | contribs) (→‎Design Notes)


ClyC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 681
    Illegal AgeI site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for expression in E. coli.

Source

Hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12.

References

Lee, Chanyoung, et al. “Development of Advanced Chimeric Endolysin to Control Multidrug-Resistant Staphylococcus Aureus through Domain Shuffling.” ACS Infectious Diseases, vol. 7, no. 8, 28 May 2021, pp. 2081–2092. https://doi.org/10.1021/acsinfecdis.0c00812.