Coding

Part:BBa_K4989000:Design

Designed by: Athanasia Arampatzi   Group: iGEM23_Thrace   (2023-10-02)
Revision as of 19:34, 2 October 2023 by Atharab (Talk | contribs) (→‎Design Notes)


Acetyl-CoA C-acetyltransferase (thiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 984
    Illegal BamHI site found at 849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal AgeI site found at 873
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.

Source

Our part was synthetically produced but the sequence comes from Coprococcus sp. L2-50 DSM.

References