Coding
Part:BBa_K4759025
Designed by: Mengrui Tao Group: iGEM23_Jiangnan-China (2023-10-02)
hemL
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.Glutamate-1-semialdehyde aminotransferase catalyzes the pyridoxal 5'-phosphate-dependent reaction which converts GSA to delta-aminolevulinate (ALA). (S)-4-amino-5-oxopentanoate <=> 5-aminolevulinate
Design Notes
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1028
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1028
Illegal NheI site found at 738 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1028
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1028
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1028
Illegal AgeI site found at 388
Illegal AgeI site found at 721 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis/heme
None |