Coding
Part:BBa_K4759024
Designed by: Mengrui Tao Group: iGEM23_Jiangnan-China (2023-10-02)
hemH
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis.
Design
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 871
Illegal AgeI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis/heme
None |