Coding
Part:BBa_K4759020
Designed by: Mengrui Tao Group: iGEM23_Jiangnan-China (2023-10-02)
hemA
the insufficient supply of heme limits the whole-cell P450 biocatalytic activity.A gene coding for glutamyl-tRNA reductase (GluTR),Glutamyl-tRNA reductase catalyzes the first step of porphyrin biosynthesis. Forms part of the pathway for the biosynthesis of 5-aminolevulinate from glutamate, known as the C5 pathway, which is used in most eubacteria, and in all archaebacteria, algae and plants. (S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) <=> H(+) + L-glutamyl-tRNA(Glu) + NADPH
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 246
Illegal BglII site found at 524 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 178
[edit]
Categories
Parameters
//function/biosynthesis/heme
None |