Coding

Part:BBa_K4759063:Design

Designed by: Junyuan Shi   Group: iGEM23_Jiangnan-China   (2023-10-02)
Revision as of 15:42, 12 October 2023 by MengruiTao (Talk | contribs)

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petH-RBS2-petF(D61R)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1179
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1179
    Illegal NotI site found at 952
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1179
    Illegal BglII site found at 1490
    Illegal BamHI site found at 1173
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Through previous experimental results, we selected ferredoxin reductase PetH (SEQ ID NO.7) and ferredoxin PetF (SEQ ID NO.8) from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP. The modeling group docked the two proteins, PetF and OleP, to obtain the hot spot residues and the corresponding mutated amino acids, and the petF genes were mutated by PCR to obtain the corresponding mutants.
Aspartic acid at site 61 of the PetF is mutated to arginine.


Source

ferredoxin reductase PetH (SEQ ID NO.7) and ferredoxin PetF (SEQ ID NO.8) from the algae (Synechocystis PCC 6803)


References