Coding
P0DTR5

Part:BBa_K4726001:Design

Designed by: Benjamin Ouellet   Group: iGEM23_ULaval   (2023-10-01)
Revision as of 23:44, 9 October 2023 by ArnaudG (Talk | contribs) (→‎Design Notes)


α-D-galactosamine galactosaminidase_Flavonifractor plautii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2998
    Illegal PstI site found at 77
    Illegal PstI site found at 571
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2998
    Illegal PstI site found at 77
    Illegal PstI site found at 571
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2998
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2998
    Illegal PstI site found at 77
    Illegal PstI site found at 571
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2998
    Illegal PstI site found at 77
    Illegal PstI site found at 571
    Illegal NgoMIV site found at 84
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 2403
    Illegal NgoMIV site found at 3153
    Illegal AgeI site found at 2106
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to clone this genetic construct onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before the NdeI site and after the BamHI site to assist with the restriction digestion.

Source

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References