DNA

Part:BBa_K4765117

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-01)
Revision as of 15:19, 1 October 2023 by Siliang Zhan (Talk | contribs)


ribozyme connected: H. ex mtSSB + SAHS 33020

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of ribozyme+T7_RBS+SAHS 33020+stem-loop and ribozyme+T7_RBS+H. ex mtSSB+stem-loop.

Usage and Biology

We introduced this part into E. coli, enabling the heterologous expression of both mtSSB and TDP proteins, to assess the combined desiccation resistance capability of these two proteins.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 880
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409


[edit]
Categories
//collections
Parameters
proteins