Part:BBa_K4765110
Twister P1 + T7_RBS + intimin-LCA fusion + stem-loop
Introduction
We’ve developed an E. coli-cyanobacteria adhesion module by transfecting intimin-LCA fusion. Intimin-LCA fusion is composed of intimin and LCA. is a lectin from Lentils that can recognize α-linked mannose residues. It is a common lectin that can specifically bind to the LPS on the surface of S. elongatus PCC7942. Intimin includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[1] , possesses the outer membrane anchoring of LCA. We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.
Usage and Biology
This biological component delivers LCA to the surface of E. coli, facilitating adhesion between E. coli, and S. elongatus PCC7942. We envision that the adhesion between cyanobacteria and E. coli can promote the exchange of substances within the biofilm, enhancing the biofilm's survivability.
Characterization
Sequencing map
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| Figure1 Sequencing map of LCA
Sequencing was performed using the two primers：T7: 5-TAATACGACTCACTATAGG-3 and Kan-F: 5-ATTCTCACCGGATTCAGT-3. |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1305
Reference
- ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a
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