RNA

Part:BBa_K4765021

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-08)
Revision as of 03:16, 8 October 2023 by Siliang Zhan (Talk | contribs) (→‎Usage and Biology)


stem-loop 1, from 10.1021/acssynbio.2c00416

contributed by Fudan iGEM 2023

Introduction

The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.[1].

Usage and Biology

In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of E. coli for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416
[edit]
Categories
//rna
Parameters
efficiency