DNA

Part:BBa_K4765105

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-09-30)
Revision as of 07:20, 2 October 2023 by YijunWang (Talk | contribs) (→‎Usage and Biology)


Twister P1 + T7_RBS + intimin-Ag3 fusion + stem-loop

contributed by Fudan iGEM 2023

Usage and Biology

Similar to BBa_K4765103(ribozyme+strong RBS+intimin-Ag2 fusion+stem-loop), we replaced Ag2 with Ag3 in order to interact with ribozyme+strong RBS+intimin-Nb3 fusion+stem-loop to create a biofilm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305


[edit]
Categories
//collections/biofilm
Parameters
protein