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Composite
Part:BBa_K4604015:Design
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-01)
Revision as of 16:31, 6 October 2023 by HannahSwientek (Talk | contribs)
piG_01b (tetR_bluB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1618
Design Notes
The start codon of the bluB was changed to ATG to act as a start for translation in E. coli. The region downstream of the tetA/B promoter was shortened (in comparison to BBa_K4604016) to counteract the leakiness.
Source
Modified piG_01a using Gibson Assembly.