Part:BBa_K4789009

The MAP3K7-LUC improved plasmids

The introduction of our experiment Our improved experiment is based upon the YiYe-China iGEM project from 2022(https://2022.igem.wiki/yiye-china/, https://parts.igem.org/Part:BBa_K4218004). The project aims to provide a plasmid sensor to detect intracellular alternative splicing for early diagnosis of myelodysplastic syndromes(MDS). The project had established a dual luciferase reporter system to monitor the alteration of RNA splicing in cells These reporters incorporate segments of both the intronic and exonic sequences of MAP3K7 or ZNF91 within the luciferase gene (Fig 1). The activity of luciferase can be used to measure the changes of intracellular splicing, so as to play an auxiliary role in the diagnosis of MDS.

          
                    Fig .1 The dual luciferase reporter system

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2459
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3661