Composite

Part:BBa_K4586029

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-29)
Revision as of 13:09, 29 September 2023 by Mohamed-Ab0elghare (Talk | contribs)


DART-V-ADAR (tissue specific switch)

Usage and Description

This composite part codes for our tissue-specific switch and consists of two elements: first, a stop codon (UAG) sensor that is designed to be complementary to a specific RNA within the target cell or tissue; in our case, our target is the auto-reactive B-cells that secrete ACPA, and the sensor is complementary to the variable chain of ACPA mRNA; second, a two-hairpin structure flanking the sensor, which is called MS2, that has binding affinity to MCP and is conjugated to the ADAR2 catalytic portion in order to enhance the sensitivity and amplify the signal of our therapeutic cargo (Cas12k).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None