Coding

Part:BBa_K4814010

Designed by: CHIEN-YUEH LIU   Group: iGEM23_PuiChing-Macau   (2023-09-28)
Revision as of 01:52, 29 September 2023 by Ginaliu (Talk | contribs)

We have used the codon optimized version of EYFP by Watcher, R. M. et al (1998). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814009.

The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date.

Wachter, R. M., Elsliger, M. A., Kallio, K., Hanson, G. T., & Remington, S. J. (1998). Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure (London, England : 1993), 6(10), 1267–1277. https://doi.org/10.1016/s0969-2126(98)00127-0

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