Regulatory

Part:BBa_K4779006:Design

Designed by: Ziqi Mi   Group: iGEM23_Nanjing-BioXstem   (2023-09-27)
Revision as of 15:12, 27 September 2023 by Mandym (Talk | contribs) (→‎References)

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UASprm1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

UASprm1 was obtained by PCR amplification using S.cerevisiae BY4741 genome as a template. The sequence is derived from -280bp to -101bp upstream of the pprm1 start codon.

References

John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895. Mark Rosenkrantz et al. Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1[J]. Current Genetics, 1994, 25(3) : 185-195.