DNA

Part:BBa_K4887001:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-BioX   (2023-08-14)
Revision as of 06:26, 5 October 2023 by Htclamm (Talk | contribs)

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IbGBSSI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1459
    Illegal BsaI.rc site found at 123
    Illegal SapI site found at 1156
    Illegal SapI.rc site found at 1225


Design Notes

In order to construct the IbGBSSI knockout system, the sequence of gene IbGBSSI must be understood clearly so that the appropriate sgRNA (BBa_K4887002) could be designed targeting for the gene IbGBSSI.


Source

AB071604(Accession umber of NCBI GenBank), from sweet potato (Ipomoea batatas) genomic sequence.


References