Composite
Part:BBa_K4585006
Designed by: Zijun Zhang Group: iGEM23_CSU-CHINA (2023-09-24)
pcDNA3.1(+)-3XHA-GAL4-VP64-NLS linearized vector
We used homologous recombination method to construct plasmids, pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector can undergo homologous recombination with GAL4 fragment (BBa_K4585001) and VP64 fragment to obtain pcDNA3.1(+)-3×HA-GAL4-VP64-NLS plasmid (BBa_K4585012).
Diagrams
Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector
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